

FemtoFiber Metabolic Imaging
Laser system for two-photon fluorescence lifetime imaging of NADH and FAD
- Simultaneous two-color emission for direct excitation of NADH and FAD
- Output 1: 780 nm, fixed and Output 2: 820 .. 960 nm, tunable
- Straighforward synchronization to TCSPC electronics
- Power modulation / beam blanking for Output 1 and 2 (optional)
- Simple, turn-key, and compact solution
To reliably perform metabolic imaging of biological tissue simultaneous acquisition of fluorescence lifetime images of NADH and FAD is required. In collaboration with Becker & Hickl TOPTICA has developed the FemtoFiber Metabolic Imaging laser system which allows for a straightforward acquisition of the NADH and FAD fluorescence quasi-simultaneously. The unique laser design offers a fixed-frequency output at 780 nm and a tunable output from 820 - 960 nm that can directly be synchronized to the TCSPC electronics. Each laser output can be independently controlled via an integrated AOM to enable laser multiplexing. The fully air-cooled, turn-key, and compact design makes this fiber laser system the perfect tool for 2P-FLIM.
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Specification
Specifications Laser Output Output #1 Output #2 Wavelength 780 nm* (+/- 10 nm) 820 - 960 nm* (tunable) Power > 500 mW > 100 mW (@820 nm) to > 300 mW (@960 nm) Pulse duration < 150 fs < 150 fs (typ. 100 fs) Repetition rate 80 MHz (others on request) Power modulation / beam blanking by AOM > 1 MHz modulation bandwidth (optional) >1 MHz modulation bandwidth (optional) Output coupling Free space Cooling Air-cooled *other wavelengths upon request - Additional Information
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Applications
- Metabolic Imaging
- 2P-FLIM
- 2P-Microscopy
- Multi-color imaging
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Literature
W. Becker et. al., Metabolic imaging by simultaneous 2-photon FLIM of NAD(P)H and FAD