Simplifying multi-photon microscopy with stress-less femtosecond lasers
FemtoFiber ultra 920
Femtosecond fiber laser
In two-photon microscopy, peak-power is brightness! If you care for the best image brightness, you need short pulses, high power, and most importantly a clean temporal pulse shape. With TOPTICAs unique Clean Pulse Technology, the FemtoFiber ultra 920 enables unmatched brightness in two-photon microscopy without unwanted heating of the sample.
Additionally, software-controlled dispersion precompensation (GDD) and an integrated AOM for power modulation, make the system extremely user-friendly.
TOPTICAs robust & reliable fiber laser technology significantly reduces the cost of ownership of your laser systems. In addition, our quiet & compact laser design minimizes noise-stress to the animals while at the same time saving valuable table space.
The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, eGFP, Eosin, GCaMP, CFP, Calcein or Venus.
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Your Benefits
Compact & Low cost of ownership
Saving table space with all-integrated robust fiber laser system.
Clean Pulse Technology
TOPTICA’s unique Clean Pulse Technology provides clean, pedestal-free pulses even after the fiber for best image brightness.
Images by Shau Poh Chong and Peter Török
User-friendly GDD
Short pulses and software-controlled dispersion precompensation (GDD) for user-friendly handling. TOPTICA’s FemtoFiber ultra provides motorized group delay dispersion (GDD) pre-compensation that can be controlled in software.
Fast power control
An integrated acousto-optic modulator (AOM) enables fast power-modulation and flyback blanking in synchronization with the beam scanner. This minimizes sample damage and photo-bleaching. Also, a fast electronic trigger output is available as reference for TCSPC in FLIM and gated detection.
For all important fluorophores
The FemtoFiber ultra 920 is ideally suited for the two-photon excitation of common fluorophores like GFP, GCaMP, RFP, mCherry, tdTomato, BFP, Alexa, NADH, FAD, auto-fluorescence or SHG-imaging.
More than 20 years of experience
With 20 years of experience developing OEM-class fiber lasers, TOPTICA is a leader in the field. We have tailored our FemtoFiber ultra series to provide customers with an industrial-grade light engine for high-end applications. Unlike bulky, alignment-sensitive Ti:Sapphire or OPO systems, TOPTICA's femtosecond fiber lasers are reliable and compact. They provide femtosecond pulses with high average power and excellent temporal and spatial beam quality.
Applications
Neuroscience
High peak power and clean pulse shape are needed for deep-tissue imaging.
Two-Photon microscopy
Two-photon excitation microscopy opens a window deep into living tissue.
Lithography & microfabrication
From semiconductor patterning and 3D microprinting to holographic optics and transparent displays, laser light is the most precise tool.
Label free & clinical imaging
Label-free imaging visualizes chemical and structural information in living cells without stains or labels.
Options
AOM
An AOM for fast modulation of the laser light can be integrated into the system. Ready to use. No complicated optics taking up valuable table space.
Pulse picker
An integrated pulse picker reduces the effective repetition rate from 80 MHz to 8 MHz (1:10).
Custom repetition rate
The custom repetition rate provides a modified oscillator with a pre-defined repetition rate between 40 and 80 MHz upon ordering.
Dual-color
With the FemtoFiber ultra dual-color option two FemtoFiber ultra are optically synchronized and allow simultaneous imaging with two different laser colors.
No laser wavelength tuning is required.
FemtoFiber ultra 460
The system has an additional SHG unit that can generate femtosecond pulses with an average power of >0.1 W at 460 nm.
Fiber delivery
Replace the free-space path between the femtosecond laser and the two-photon microscope. Our product FemtoFiber ultra FD delivers its pulses via a polarization-maintaining hollow-core fiber.
