Two-photon fluorescence lifetime imaging (FLIM) can provide insights beyond standard fluorescence microscopy and non-linear microscopy by analyzing fluorescence decay times. In addition, FLIM can be used as a label-free, autofluorescence technique and is therefore non-invasive. For example, simultaneous NADH and FAD decay measurements provide insight into the metabolic state of biological samples.

TOPTICA's offer

TOPTICA’s femtosecond fiber lasers are easy to use and offer optimal performance. In addition, a fast electronic trigger output serves as a reference for time-correlated single photon counting (TCSPC) electronics in fluorescence lifetime imaging (FLIM) and gated detectors.